Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (MCAD) (N-Term) antibody

Catalog No. ABIN2777593

The Rabbit Polyclonal anti-Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial antibody is suitable to detect Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial in samples from Human, Mouse, Rat, Cow and Horse. It has been validated for WB.

Quick Overview for Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (MCAD) (N-Term) antibody (ABIN2777593)

Target: Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (MCAD)

Reactivity: Human, Mouse, Rat, Cow, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Binding Specificity: N-Term

Sequence: AAGFGRCCRV LRSISRFHWR SQHTKANRQR EPGLGFSFEF TEQQKEFQAT

Predicted Reactivity: Cow: 92%, Horse: 92%, Human: 100%, Mouse: 91%, Rat: 91%

Characteristics: This is a rabbit polyclonal antibody against ACADM. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ACADM

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 421 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details

Target: Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (MCAD)

Alternative Name: ACADM

Background: ACADM Is the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Clinical phenotypes are associated with ACADM hereditary deficiency.This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: ACAD1, MCAD, MCADH
Protein Interaction Partner: SUMO2, SUMO3, UBC, MDM2, STRAP, HDAC1, UBD, CUL3, SUMO4, CALM1, USP50, USP20, ACADM,
Protein Size: 421

Molecular Weight: 46 kDa

Gene ID: 34

NCBI Accession: NM_000016, NP_000007

UniProt: P11310