SIAH2 antibody (Middle Region)

Catalog No. ABIN2777843

This Rabbit Polyclonal antibody specifically detects SIAH2 in WB. It exhibits reactivity toward Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Horse and Rabbit.

Quick Overview for SIAH2 antibody (Middle Region) (ABIN2777843)

Target: SIAH2 (Siah E3 Ubiquitin Protein Ligase 2 (SIAH2))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIAH2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SIAH2 Antibody

Binding Specificity: Middle Region

Sequence: AVDWVMMQSC FGHHFMLVLE KQEKYEGHQQ FFAIVLLIGT RKQAENFAYR

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against SIAH2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SIAH2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 324 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SIAH2

Target: SIAH2 (Siah E3 Ubiquitin Protein Ligase 2 (SIAH2))

Alternative Name: SIAH2

Background: SIAH2 is a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in regulating cellular response to hypoxia.This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in regulating cellular response to hypoxia. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: hSiah2
Protein Interaction Partner: EGLN3, XAF1, HIPK2, UBE2D1, UBE2L6, SIAH2, UBE2D3, UBC, PPP1R13B, TP53BP2, NFE2L2, USP19, AR, UBE2I, STAT3, MYD88, EEF1D, CCBE1, SETD2, HIPK3, TNK2, KAT5, KAT2B, UBE2D2, TPX2, PML, ATN1, PSMD4, PPP1R12A, EGLN2, SH3RF1, EGLN1, DYRK2, PPP1CC, AFF1, DCC, CEB
Protein Size: 324

Molecular Weight: 34 kDa

Gene ID: 6478

NCBI Accession: NM_005067, NP_005058

UniProt: O43255