MRPS15 antibody (N-Term)

Catalog No. ABIN2777848

The Rabbit Polyclonal anti-MRPS15 antibody has been validated for WB and IHC. It is suitable to detect MRPS15 in samples from Human, Mouse, Rat, Cow, Guinea Pig, Rabbit, Horse and Dog. There is 1 publication available.

Quick Overview for MRPS15 antibody (N-Term) (ABIN2777848)

Target: MRPS15 (Mitochondrial Ribosomal Protein S15 (MRPS15))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Rabbit, Horse, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPS15 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-MRPS15 Antibody

Binding Specificity: N-Term

Sequence: RGYVVRKPAQ SRLDDDPPPS TLLKDYQNVP GIEKVDDVVK RLLSLEMANK

Predicted Reactivity: Cow: 100%, Dog: 92%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 83%, Rabbit: 86%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against MRPS15. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MRPS15

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 257 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-MRPS15 antibody (ABIN2777848)

Davies, Rackham, Shearwood, Hamilton, Narsai, Whelan, Filipovska: "Pentatricopeptide repeat domain protein 3 associates with the mitochondrial small ribosomal subunit and regulates translation." in: FEBS letters, Vol. 583, Issue 12, pp. 1853-8, (2009) (PubMed).

Target Details for MRPS15

Target: MRPS15 (Mitochondrial Ribosomal Protein S15 (MRPS15))

Alternative Name: MRPS15

Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRPS15 is a 28S subunit protein that belongs to the ribosomal protein S15P family.Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S15P family. The encoded protein is more than two times the size of its E. coli counterpart, with the 12S rRNA binding sites conserved. Between human and mouse, the encoded protein is the least conserved among small subunit ribosomal proteins. Pseudogenes corresponding to this gene are found on chromosomes 15q and 19q.
Alias Symbols: DC37, FLJ11564, MPR-S15, RPMS15, S15mt
Protein Interaction Partner: GRSF1, UBC, PARK2, ESR2, PTCD3, CAND1, COPS5, CUL3, SUMO2, ICT1,
Protein Size: 257

Molecular Weight: 30 kDa

Gene ID: 64960

NCBI Accession: NM_031280, NP_112570

UniProt: P82914