BAF53A antibody (N-Term)

Catalog No. ABIN2777881

This anti-BAF53A antibody is a Rabbit Polyclonal antibody detecting BAF53A in WB. Suitable for Human, Mouse, Rat, Cow, Dog, Horse, Rabbit and Guinea Pig.

Quick Overview for BAF53A antibody (N-Term) (ABIN2777881)

Target: BAF53A (Actin-Like 6A (BAF53A))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAF53A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BAF53A Antibody

Binding Specificity: N-Term

Sequence: VDFPTAIGMV VERDDGSTLM EIDGDKGKQG GPTYYIDTNA LRVPRENMEA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against ACTL6A. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ACTL6A

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 429 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for BAF53A

Target: BAF53A (Actin-Like 6A (BAF53A))

Alternative Name: ACTL6A

Background: This gene encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila, the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described.
Alias Symbols: ACTL6, BAF53A, MGC5382, Arp4, INO80K, ARPN-BETA
Protein Interaction Partner: UBC, BMI1, HECW2, FBXO6, HDAC2, CBX5, UCHL5, ARID1B, RUVBL1, TRRAP, ARID1A, SMARCE1, SMARCC2, SMARCC1, SMARCA4, SMARCA2, UBD, GRB2, INO80E, CAND1, CUL3, CDK9, CDK2, H2AFZ, TP53, UL27, SMARCD1, HDGF, SOX2, SMARCAD1, KAT5, PBRM1, Bcl7b, INO80C, ACTR8, INO80
Protein Size: 429

Molecular Weight: 47 kDa

Gene ID: 86

NCBI Accession: NM_004301, NP_004292

UniProt: O96019

Pathways: Chromatin Binding, Photoperiodism