Cyclin D1 antibody (N-Term)

Catalog No. ABIN2777893

Product Details anti-Cyclin D1 Antibody

Target: Cyclin D1 (CCND1)

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Guinea Pig, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin D1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: KCEEEVFPLA MNYLDRFLSL EPVKKSRLQL LGATCMFVAS KMKETIPLTA

Predicted Reactivity: Cow: 100%, Dog: 92%, Guinea Pig: 92%, Human: 100%, Mouse: 92%, Pig: 100%, Rabbit: 92%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against CCND1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CCND1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 295 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Cyclin D1

Target: Cyclin D1 (CCND1)

Alternative Name: CCND1 (CCND1 Products)

Background: CCND1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: BCL1, D11S287E, PRAD1, U21B31
Protein Interaction Partner: CDKN1B, CDKN1A, CDK4, RELA, FBXO31, UBC, FBXO4, CAMK1, TP73, RB1, CDK2, HSPA8, POLR1B, MCM10, ORC4, LPL, BRINP1, CDK6, RABEP1, CARD11, CUL1, RBL2, UBTF, CCND1, ATF2, CDKN2A, FBXW8, BTRC, PPP1CC, PPP1CB, PSMA6, ITLN1, MCM7, MCM6, MCM3, MCM2, ELAVL1, PCNA,
Protein Size: 295

Molecular Weight: 34 kDa

Gene ID: 595

NCBI Accession: NM_053056, NP_444284

UniProt: P24385

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling