T Brachyury Protein (Middle Region) antibody

Catalog No. ABIN2777907

This Rabbit Polyclonal antibody specifically detects in WB. It exhibits reactivity toward Human, Mouse, Cow, Dog, Rat, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio).

Quick Overview for T Brachyury Protein (Middle Region) antibody (ABIN2777907)

Target: T Brachyury Protein

Reactivity: Human, Mouse, Cow, Dog, Rat, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Binding Specificity: Middle Region

Sequence: TSTLCPPANP HPQFGGALSL PSTHSCDRYP TLRSHRSSPY PSPYAHRNNS

Predicted Reactivity: Cow: 83%, Dog: 100%, Guinea Pig: 92%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%, Zebrafish: 82%

Characteristics: This is a rabbit polyclonal antibody against T. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human T

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 435 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details

Target: T Brachyury Protein

Background: T protein is involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. It binds to a palindromic site (called T site) and activates gene transcription when bound to such a site.The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.
Alias Symbols: MGC104817, TFT
Protein Size: 435

Molecular Weight: 47 kDa

Gene ID: 6862

NCBI Accession: NM_003181, NP_003172

UniProt: O15178

Pathways: Tube Formation