TEF antibody (Middle Region)

Catalog No. ABIN2777938

The Rabbit Polyclonal anti-TEF antibody has been validated for WB. It is suitable to detect TEF in samples from Human, Mouse, Rat, Dog, Cow, Sheep, Horse and Rabbit.

Quick Overview for TEF antibody (Middle Region) (ABIN2777938)

Target: TEF (Thyrotrophic Embryonic Factor (TEF))

Reactivity: Human, Mouse, Rat, Dog, Cow, Sheep, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TEF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TEF Antibody

Binding Specificity: Middle Region

Sequence: RKDEGRKEAA EGKEQGLAPL VVQTDSASPL GAGHLPGLAF SSHLHGQQFF

Predicted Reactivity: Cow: 92%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 92%, Rabbit: 85%, Rat: 92%, Sheep: 92%

Characteristics: This is a rabbit polyclonal antibody against TEF. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TEF

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 303 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for TEF

Target: TEF (Thyrotrophic Embryonic Factor (TEF))

Alternative Name: TEF

Background: TEF (thyrotroph embryonic factor) is a member of the PAR bZip (proline and acidic amino acid-rich basic leucine zipper) transcription factor family. It accumulates with robust circadian rhythms in tissues with high amplitudes of clock gene expression. Thyrotroph embryonic factor (TEF), a transcription factor, is a member of the PAR (proline and acidic amino acid-rich) subfamily of basic region/leucine zipper (bZIP) transcription factors. It is expressed in a broad range of cells and tissues in adult animals, however, during embryonic development, TEF expression appears to be restricted to the developing anterior pituitary gland, coincident with the appearance of thyroid-stimulating hormone, beta (TSHB). Indeed, TEF can bind to, and transactivate the TSHB promoter. It shows homology (in the functional domains) with other members of the PAR-bZIP subfamily of transcription factors, which include albumin D box-binding protein (DBP), human hepatic leukemia factor (HLF) and chicken vitellogenin gene-binding protein (VBP), VBP is considered the chicken homologue of TEF. Different members of the subfamily can readily form heterodimers, and share DNA-binding, and transcriptional regulatory properties.
Alias Symbols: -
Protein Interaction Partner: MRPL28, DBP,
Protein Size: 303

Molecular Weight: 33 kDa

Gene ID: 7008

NCBI Accession: NM_003216, NP_003207

UniProt: Q10587