Claudin 5 antibody (C-Term)

Catalog No. ABIN2778047

This Rabbit Polyclonal antibody specifically detects Claudin 5 in WB. It exhibits reactivity toward Human, Rat, Mouse, Pig, Cow, Rabbit and Guinea Pig and has been mentioned in 2+ publications.

Quick Overview for Claudin 5 antibody (C-Term) (ABIN2778047)

Target: Claudin 5 (CLDN5)

Reactivity: Human, Rat, Mouse, Pig, Cow, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Claudin 5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Claudin 5 Antibody

Binding Specificity: C-Term

Sequence: GWAATALLMV GGCLLCCGAW VCTGRPDLSF PVKYSAPRRP TATGDYDKKN

Predicted Reactivity: Cow: 93%, Guinea Pig: 86%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against CLDN5. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human CLDN5

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 218 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Claudin 5 antibody (ABIN2778047)

Yang, Liu, Miao, Yang, Fu, Dou, Cai, Zong, Tan, Chen, Wang: "Induction of VEGFA and Snail-1 by meningitic Escherichia coli mediates disruption of the blood-brain barrier." in: Oncotarget, Vol. 7, Issue 39, pp. 63839-63855, (2016) (PubMed).

Fontijn, Volger, Fledderus, Reijerkerk, de Vries, Horrevoets: "SOX-18 controls endothelial-specific claudin-5 gene expression and barrier function." in: American journal of physiology. Heart and circulatory physiology, Vol. 294, Issue 2, pp. H891-900, (2008) (PubMed).

Target Details for Claudin 5

Target: Claudin 5 (CLDN5)

Alternative Name: CLDN5

Background: CLDN5 is a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome.This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: AWAL, BEC1, CPETRL1, TMVCF
Protein Interaction Partner: UBC, MPDZ, CLDN3, CLDN5, TJP1, CLDN1,
Protein Size: 218

Molecular Weight: 23 kDa

Gene ID: 7122

NCBI Accession: NM_003277, NP_003268

UniProt: O00501

Pathways: Hepatitis C