CD40 Ligand antibody (Middle Region)

Catalog No. ABIN2778145

The Rabbit Polyclonal anti-CD40 Ligand antibody has been validated for WB and IHC. It is suitable to detect CD40 Ligand in samples from Human, Mouse, Rat, Pig, Cow, Dog, Rabbit, Sheep and Horse.

Quick Overview for CD40 Ligand antibody (Middle Region) (ABIN2778145)

Target: CD40 Ligand (CD40LG)

Reactivity: Human, Mouse, Rat, Pig, Cow, Dog, Rabbit, Sheep, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD40 Ligand antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CD40 Ligand Antibody

Binding Specificity: Middle Region

Sequence: ENSFEMQKGD QNPQIAAHVI SEASSKTTSV LQWAEKGYYT MSNNLVTLEN

Predicted Reactivity: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 83%, Pig: 100%, Rabbit: 92%, Rat: 85%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against CD40LG. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CD40LG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 261 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for CD40 Ligand

Target: CD40 Ligand (CD40LG)

Alternative Name: CD40LG

Background: CD40LG is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-1375 BC071754.1 1-1375 1376-1599 X67878.1 1349-1572 1600-1834 BC071754.1 1596-1830
Alias Symbols: CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L
Protein Interaction Partner: UBC, TRAF2, TRAF1, CD40, BIRC3, BIRC2, CRYAB, IGHG1, CD5L, CD40LG, TP53, IGKC, RNF128, HPR, APOA1,
Protein Size: 261

Molecular Weight: 29 kDa

Gene ID: 959

NCBI Accession: NM_000074, NP_000065

UniProt: P29965

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints