SUPT5H antibody (Middle Region)

Catalog No. ABIN2778245

The Rabbit Polyclonal anti-SUPT5H antibody has been validated for WB. It is suitable to detect SUPT5H in samples from Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig and Zebrafish (Danio rerio). There are 2+ publications available.

Quick Overview for SUPT5H antibody (Middle Region) (ABIN2778245)

Target: SUPT5H (Transcription Elongation Factor SPT5 (SUPT5H))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUPT5H antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SUPT5H Antibody

Binding Specificity: Middle Region

Sequence: FEDQPEGIDL EVVTESTGKE REHNFQPGDN VEVCEGELIN LQGKILSVDG

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against SUPT5H. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SUPT5H

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1087 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-SUPT5H antibody (ABIN2778245)

Ueki, Lin, Senoo, Ebihara, Agata, Onji, Saheki, Kawai, Mukherjee, Reichenberger, Olsen: "Increased myeloid cell responses to M-CSF and RANKL cause bone loss and inflammation in SH3BP2 "cherubism" mice." in: Cell, Vol. 128, Issue 1, pp. 71-83, (2007) (PubMed).

Amir-Zilberstein, Ainbinder, Toube, Yamaguchi, Handa, Dikstein: "Differential regulation of NF-kappaB by elongation factors is determined by core promoter type." in: Molecular and cellular biology, Vol. 27, Issue 14, pp. 5246-59, (2007) (PubMed).

Target Details for SUPT5H

Target: SUPT5H (Transcription Elongation Factor SPT5 (SUPT5H))

Alternative Name: SUPT5H

Background: SUPT5H and its binding partner regulate transcriptional elongation by RNA polymerase II. SPT4 and SPT5 are involved in both 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB)-mediated transcriptional inhibition and the activation of transcriptional elongation by the human immunodeficiency virus type 1 (HIV-1) Tat protein.
Alias Symbols: FLJ34157, SPT5, SPT5H, Tat-CT1
Protein Interaction Partner: POLR2A, BRCA1, UBC, RNF2, SAMHD1, GANAB, SWAP70, PAICS, CARM1, FARSB, SEC24C, RANBP3, CSDE1, SF1, SET, PLOD2, MSH2, MSH6, GART, XRCC6, ATP6V1B2, SOX2, LMNA, CDK9, CCNT2, CCNT1, tat, NSMF, FN1, CSNK2A1, SIK1, ZNF512B, LMAN2, ZFYVE9, SNX4, SUPT4H1, POLR2C,
Protein Size: 1087

Molecular Weight: 121 kDa

Gene ID: 6829

NCBI Accession: NM_003169, NP_003160

UniProt: O00267