MED6 antibody (Middle Region)

Catalog No. ABIN2778336

This anti-MED6 antibody is a Rabbit Polyclonal antibody detecting MED6 in WB and ChIP. Suitable for Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig and Pig.

Quick Overview for MED6 antibody (Middle Region) (ABIN2778336)

Target: MED6 (Mediator Complex Subunit 6 (MED6))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED6 antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Product Details anti-MED6 Antibody

Binding Specificity: Middle Region

Sequence: LLLDLRQKFP PKFVQLKPGE KPVPVDQTKK EAEPIPETVK PEEKETTKNV

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 86%, Pig: 93%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against MED6. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MED6

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 246 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MED6

Target: MED6 (Mediator Complex Subunit 6 (MED6))

Alternative Name: MED6

Background: MED6 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. And mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Alias Symbols: NY-REN-28
Protein Interaction Partner: TARDBP, UBC, CDK19, CDK8, MED10, MED23, CCNC, MED19, MED26, FBXW7, MED11, MED28, MED29, MED4, MED12, MED7, MED21, MED1, SMARCA4, BCL6, SUZ12, CUL1, SKP1, CTDP1, MED25, SREBF1, MED15, VDR, OBFC1, MED18, ZC3H13, TADA2A, RARA, PARP1, SMAD3, SMAD2, SMAD1, MED
Protein Size: 246

Molecular Weight: 28 kDa

Gene ID: 10001

NCBI Accession: NM_005466, NP_005457

UniProt: O75586

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha