OCT4 antibody (Middle Region)

Catalog No. ABIN2778604

This Rabbit Polyclonal antibody specifically detects OCT4 in WB. It exhibits reactivity toward Human, Mouse, Rat, Pig, Cow, Dog, Rabbit, Goat, Horse, Sheep and Guinea Pig.

Quick Overview for OCT4 antibody (Middle Region) (ABIN2778604)

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reactivity: Human, Mouse, Rat, Pig, Cow, Dog, Rabbit, Goat, Horse, Sheep, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OCT4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-OCT4 Antibody

Binding Specificity: Middle Region

Sequence: LLQKWVEEAD NNENLQEICK AETLVQARKR KRTSIENRVR GNLENLFLQC

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 79%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 93%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against POU5F1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human POU5F1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 265 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Alternative Name: POU5F1

Background: POU5F1 is a POU transcription factor expressed by early embryo cells and germ cells. It determines paracrine growth factor signaling from stem cells to the trophectoderm.
Alias Symbols: MGC22487, OCT3, OCT4, OTF3, OTF4, OTF-3, Oct-3, Oct-4
Protein Interaction Partner: ETS2, CETN2, XPC, RAD23B, UBC, ITCH, FOXD3, WWP2, PSMD10, NCL, PURA, PPIA, POU5F1, YBX1, KRT19, KRT14, KRT10, KRT9, KRT2, KRT1, HNRNPK, HMGB1, HIST1H1T, GAPDH, FKBP7, EHMT2, AGR2, TUBB4A, EIF5B, TUBA1A, NR2F2, TRA2B, SRSF3, RPS25, RPS23, RPS19, RPL31, RPL
Protein Size: 265

Molecular Weight: 30 kDa

Gene ID: 5460

NCBI Accession: NM_203289, NP_976034

Pathways: Stem Cell Maintenance