beta Actin antibody (Middle Region)

Catalog No. ABIN2778644

This anti-beta Actin antibody is a Rabbit Polyclonal antibody detecting beta Actin in WB and IHC. Suitable for Human, Mouse, Rat, Pig, Cow, Sheep, Zebrafish (Danio rerio), Horse and Goat. This Primary Antibody has been cited in 2+ publications.

Quick Overview for beta Actin antibody (Middle Region) (ABIN2778644)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human, Mouse, Rat, Pig, Cow, Sheep, Zebrafish (Danio rerio), Horse, Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-beta Actin Antibody

Binding Specificity: Middle Region

Sequence: TMKIKIIAPP ERKYSVWIGG SILASLSTFQ QMWISKQEYD ESGPSIVHRK

Predicted Reactivity: Cow: 100%, Goat: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against ACTB. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Human ACTB

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 375 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and may contain up to 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-beta Actin antibody (ABIN2778644)

Phungphong, Kijtawornrat, Wattanapermpool, Bupha-Intr: "Regular exercise modulates cardiac mast cell activation in ovariectomized rats." in: The journal of physiological sciences : JPS, Vol. 66, Issue 2, pp. 165-73, (2016) (PubMed).

Rinne, Clements, Lamme, Duijf, Bolat, Meijer, Scheffer, Rosser, Tan, McGrath, Schalkwijk, Brunner, Zhou, van Bokhoven: "A novel translation re-initiation mechanism for the p63 gene revealed by amino-terminal truncating mutations in Rapp-Hodgkin/Hay-Wells-like syndromes." in: Human molecular genetics, Vol. 17, Issue 13, pp. 1968-77, (2008) (PubMed).

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: ACTB

Background: This protein is one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Alias Symbols: BRWS1, PS1TP5BP1
Protein Interaction Partner: FUS, DSTN, CAP2, CFL2, CFL1, ACTG1, ACTB, PA2G4, EHHADH, WDYHV1, HDAC6, ISG15, TP53, CEP250, TUBGCP2, TUBGCP3, VCP, UBC, TUBG1, SUMO2, LGALS3BP, AMOT, NEDD1, HAUS1, IVNS1ABP, STAU1, MDM2, CORO7, LGR4, ASB12, ASB9, ZBTB1, ERG, RPA3, RPA2, RPA1, CDKN2A, PAR
Protein Size: 375

Molecular Weight: 42 kDa

Gene ID: 60

NCBI Accession: NM_001101, NP_001092

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction