PRMT5 antibody (N-Term)

Catalog No. ABIN2778654

The Rabbit Polyclonal anti-PRMT5 antibody has been validated for WB and IHC. It is suitable to detect PRMT5 in samples from Human, Mouse, Rat, Dog, Horse, Rabbit, Cow, Guinea Pig and Zebrafish (Danio rerio).

Quick Overview for PRMT5 antibody (N-Term) (ABIN2778654)

Target: PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))

Reactivity: Human, Mouse, Rat, Dog, Horse, Rabbit, Cow, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRMT5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PRMT5 Antibody

Binding Specificity: N-Term

Sequence: FDFLCMPVFH PRFKREFIQE PAKNRPGPQT RSDLLLSGRD WNTLIVGKLS

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 77%

Characteristics: This is a rabbit polyclonal antibody against PRMT5. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PRMT5

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 620 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PRMT5

Target: PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))

Alternative Name: PRMT5

Background: PRMT5 methylates specific arginine residues in the small nuclear ribonucleoproteins Sm D1 and Sm D3 to monomethylarginine and to symmetrical dimethylarginines (sDMAs). It methylates SUPT5H. PRMT5 plays a role in the assembly of snRNP core particles and may play a role in cytokine-activated transduction pathways. It negatively regulates cyclin E1 promoter activity and cellular proliferation and May regulate the SUPT5H transcriptional elongation properties. It may be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. PRMT5 methylates histone H2A/H4 'Arg-3' during germ cell development and methylates histone H3 'Arg-8', which may repress transcription.
Alias Symbols: JBP1, SKB1, IBP72, SKB1Hs, HRMT1L5
Protein Interaction Partner: UBC, TP53, ING2, PRDM1, NFE4, WDR5, SUMO2, WWOX, CDYL2, LENG8, LDHAL6B, PRPF38A, ING5, ZNF436, WDR77, GRHL3, TRIB3, RSRP1, LUC7L, RBM23, GTPBP2, H3F3C, FOXK1, ZUFSP, FAM76B, NUDCD2, MAGEB2, NELFCD, COPS4, COPS7A, DCAF8, SPAG8, SLU7, MCRS1, CUL4A, ZNF2, YW
Protein Size: 620

Molecular Weight: 68 kDa

Gene ID: 10419

NCBI Accession: NM_001039619, NP_001034708

UniProt: O14744

Pathways: Chromatin Binding, Regulation of Muscle Cell Differentiation, Ribonucleoprotein Complex Subunit Organization, Skeletal Muscle Fiber Development