GAPDH antibody (C-Term)

Catalog No. ABIN2778656

The Rabbit Polyclonal anti-GAPDH antibody has been validated for WB. It is suitable to detect GAPDH in samples from Human, Mouse, Rat, Rabbit, Dog, Cow, Horse and Guinea Pig.

Quick Overview for GAPDH antibody (C-Term) (ABIN2778656)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Cow, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GAPDH Antibody

Binding Specificity: C-Term

Sequence: FNSNSHSSTF DAGAGIALND NFVKLISWYD NEYGYSNRVV DLMAYMASKE

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against Gapdh. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Mouse Gapdh

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 333 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: Gapdh

Background: Gapdh has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Gapdh also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC.
Alias Symbols: Gapd, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239
Protein Interaction Partner: Isg15, Kcnma1, Klhl40, Eed, Fbxo32, Lipc, Nanog, Siah1a, Ywhae,
Protein Size: 333

Molecular Weight: 36 kDa

Gene ID: 14433

NCBI Accession: NM_008084, NP_032110

UniProt: P16858