EIF4B antibody (Middle Region)

Catalog No. ABIN2778772

This anti-EIF4B antibody is a Rabbit Polyclonal antibody detecting EIF4B in WB. Suitable for Human, Mouse, Rat, Horse, Cow, Guinea Pig, Rabbit and Dog.

Quick Overview for EIF4B antibody (Middle Region) (ABIN2778772)

Target: EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

Reactivity: Human, Mouse, Rat, Horse, Cow, Guinea Pig, Rabbit, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EIF4B Antibody

Binding Specificity: Middle Region

Sequence: QTGNSSRGPG DGGNRDHWKE SDRKDGKKDQ DSRSAPEPKK PEENPASKFS

Predicted Reactivity: Cow: 86%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 86%, Rabbit: 92%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against EIF4B. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human EIF4B

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 611 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for EIF4B

Target: EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

Alternative Name: EIF4B

Background: IF4B contains 1 RRM (RNA recognition motif) domain and is required for the binding of mRNA to ribosomes. Functions of EIF4B are in close association with EEF4-F and EIF4-A. It binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP and promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Alias Symbols: EIF-4B, PRO1843
Protein Interaction Partner: UBC, ASB2, ZBTB1, SAMHD1, XPO7, SEC23A, PRMT3, CUL4B, USP11, SF1, XRCC5, OSBP, NARS, Stk11, CTNNA1, PARK2, FBXO6, PAN2, CSNK2A1, EIF4A3, YWHAE, MAGOH, SMURF1, NUP107, PHAX, SLC4A1AP, CCT6B, NUP153, POLR1C, RPL29, MYH9, EIF4G2, EIF4G1, EIF4A1, CSTF2, CUL3,
Protein Size: 611

Molecular Weight: 69 kDa

Gene ID: 1975

NCBI Accession: NM_001417, NP_001408

UniProt: P23588