EIF4G2 antibody (N-Term)

Catalog No. ABIN2778774

The Rabbit Polyclonal anti-EIF4G2 antibody has been validated for WB and IHC. It is suitable to detect EIF4G2 in samples from Human, Mouse, Rat, Cow, Rabbit, Dog, Horse and Guinea Pig.

Quick Overview for EIF4G2 antibody (N-Term) (ABIN2778774)

Target: EIF4G2 (Eukaryotic Translation Initiation Factor 4 gamma 2 (EIF4G2))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4G2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-EIF4G2 Antibody

Binding Specificity: N-Term

Sequence: PNFDGPAAEG QPGQKQSTTF RRLLISKLQD EFENRTRNVD VYDKRENPLL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against EIF4G2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human EIF4G2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 907 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for EIF4G2

Target: EIF4G2 (Eukaryotic Translation Initiation Factor 4 gamma 2 (EIF4G2))

Alternative Name: EIF4G2

Background: Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. EIF4G2 shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3, eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, EIF4G2 functions as a general repressor of translation by forming translationally inactive complexes. Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3, eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.
Alias Symbols: AAG1, DAP5, FLJ41344, NAT1, p97, P97
Protein Interaction Partner: SUMO2, UBC, SKIL, RPA2, ITGA4, FN1, Dlg4, POLDIP3, SLIRP, EIF4G3, RAB7A, EIF4H, EIF4G1, EIF4B, EIF4A1, DCUN1D1, EIF3F, MKNK1, EIF2S2, EIF3A, EIF5, EIF4A2, CASP3, NCBP1, EIF4E, MAGEA6,
Protein Size: 907

Molecular Weight: 102 kDa

Gene ID: 1982

NCBI Accession: NM_001418, NP_001409

UniProt: P78344