AARS antibody (N-Term)

Catalog No. ABIN2778779

The Rabbit Polyclonal anti-AARS antibody has been validated for WB and IHC. It is suitable to detect AARS in samples from Human, Rat, Mouse, Guinea Pig, Horse, Cow, Rabbit, Zebrafish (Danio rerio) and Dog.

Quick Overview for AARS antibody (N-Term) (ABIN2778779)

Target: AARS (Alanyl tRNA Synthetase (AARS))

Reactivity: Human, Rat, Mouse, Guinea Pig, Horse, Cow, Rabbit, Zebrafish (Danio rerio), Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AARS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-AARS Antibody

Binding Specificity: N-Term

Sequence: DSTLTASEIR QRFIDFFKRN EHTYVHSSAT IPLDDPTLLF ANAGMNQFKP

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 86%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against AARS. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human AARS

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 968 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for AARS

Target: AARS (Alanyl tRNA Synthetase (AARS))

Alternative Name: AARS

Background: The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41 % identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41 % identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure.
Alias Symbols: CMT2N
Protein Interaction Partner: ISG15, UBC, SUMO2, SUMO1, NEDD8, TPD52, STAT5B, SRP9, PPP2R5E, METTL1, HSF1, CALU, DPP9, NIF3L1, ANKMY2, DPP8, IPO11, EEF2K, CACYBP, ARPC1B, SAE1, TOM1L1, EIF4A3, WARS, TPD52L2, UBD, VCAM1, ITGA4, HSP90AB1, HSP90AA1, FN1, NSUN2, LARS, FARSB, UBA1, QARS, N
Protein Size: 968

Molecular Weight: 107 kDa

Gene ID: 16

NCBI Accession: NM_001605, NP_001596

UniProt: P49588