Aconitase 1 antibody (N-Term)

Catalog No. ABIN2778793

The Rabbit Polyclonal anti-Aconitase 1 antibody has been validated for WB and IHC. It is suitable to detect Aconitase 1 in samples from Human, Mouse, Rat, Cow, Rabbit, Dog, Guinea Pig, Horse and Pig.

Quick Overview for Aconitase 1 antibody (N-Term) (ABIN2778793)

Target: Aconitase 1 (ACO1)

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Guinea Pig, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aconitase 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Aconitase 1 Antibody

Binding Specificity: N-Term

Sequence: MSNPFAHLAE PLDPVQPGKK FFNLNKLEDS RYGRLPFSIR VLLEAAIRNC

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 91%, Horse: 92%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 86%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against ACO1. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ACO1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 889 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Aconitase 1

Target: Aconitase 1 (ACO1)

Alternative Name: ACO1

Background: ACO1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). It plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes.Aconitase 1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). IREs are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA. Thus, IREB1 plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes.
Alias Symbols: IRP1, ACONS, IREB1, IREBP, IREBP1
Protein Interaction Partner: UBC, FBXL5, DCPS, STRAP, FUBP1, PSMG1, HIST1H2AB, LGALS1, CTPS1, C12orf57, APP, AKT1,
Protein Size: 889

Molecular Weight: 98 kDa

Gene ID: 48

NCBI Accession: NM_002197, NP_002188

UniProt: P28271

Pathways: Transition Metal Ion Homeostasis