EIF4G3 antibody (Middle Region)

Catalog No. ABIN2778868

The Rabbit Polyclonal anti-EIF4G3 antibody has been validated for WB. It is suitable to detect EIF4G3 in samples from Human, Mouse, Rat, Cow, Horse, Rabbit, Guinea Pig and Dog. There is 1 publication available.

Quick Overview for EIF4G3 antibody (Middle Region) (ABIN2778868)

Target: EIF4G3 (Eukaryotic Translation Initiation Factor 4 gamma 3 (EIF4G3))

Reactivity: Human, Mouse, Rat, Cow, Horse, Rabbit, Guinea Pig, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4G3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EIF4G3 Antibody

Binding Specificity: Middle Region

Sequence: MRGGSSKDLL DNQSQEEQRR EMLETVKQLT GGVDVERNST EAERNKTRES

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against EIF4G3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human EIF4G3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1585 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-EIF4G3 antibody (ABIN2778868)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for EIF4G3

Target: EIF4G3 (Eukaryotic Translation Initiation Factor 4 gamma 3 (EIF4G3))

Alternative Name: EIF4G3

Background: EIF4G3 is probable component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. It is thought to be a functional homolog of EIF4G1.
Alias Symbols: eIF4GII, eIF4G 3, eIF-4G 3
Protein Interaction Partner: UBC, LGR4, TARDBP, ESR1, LIG4, EIF4G2, EIF4G1, EIF4A2, EIF4A1, SIRT7, VAV2, MKNK1, USP10, EIF3H, EIF3F, CAMK1, PABPC1, EIF4E,
Protein Size: 1585

Molecular Weight: 174 kDa

Gene ID: 8672

NCBI Accession: NM_003760, NP_003751

UniProt: O43432