EIF2S1 antibody (C-Term)

Catalog No. ABIN2778883

The Rabbit Polyclonal anti-EIF2S1 antibody has been validated for WB and IHC. It is suitable to detect EIF2S1 in samples from Human, Mouse, Rat, Cow, Dog, Zebrafish (Danio rerio), Rabbit, Guinea Pig, Horse, Goat and Saccharomyces cerevisiae.

Quick Overview for EIF2S1 antibody (C-Term) (ABIN2778883)

Target: EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Zebrafish (Danio rerio), Rabbit, Guinea Pig, Horse, Goat, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF2S1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-EIF2S1 Antibody

Binding Specificity: C-Term

Sequence: RGVFNVQMEP KVVTDTDETE LARQMERLER ENAEVDGDDD AEEMEAKAED

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 79%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against EIF2S1. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human EIF2S1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 315 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for EIF2S1

Target: EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))

Alternative Name: EIF2S1

Background: The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD), and gamma (52 kD). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha.The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987).[supplied by OMIM].
Alias Symbols: EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A
Protein Interaction Partner: HUWE1, UBC, SUMO2, SUMO3, RPA3, RPA2, RPA1, RNF2, EIF2S3, DARS, RPS27L, EEF1E1, AIMP1, EIF2S2, RPLP0, RPL27A, RPL15, RPL13, RPL7A, QARS, MARS, MAP4, TERT, NPM1, MMS19, Dlg4, VCAM1, UPF1, ITGA4, FN1, CSNK2A1, EIF2AK2, NCOA5, POP1, STRN, YBX1, DDX1, TMED9,
Protein Size: 315

Molecular Weight: 35 kDa

Gene ID: 1965

NCBI Accession: NM_004094, NP_004085

UniProt: P05198

Pathways: Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C