HNRNPM antibody (N-Term)

Catalog No. ABIN2778962

This Rabbit Polyclonal antibody specifically detects HNRNPM in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Pig, Dog, Horse, Rabbit and Guinea Pig and has been mentioned in 2+ publications.

Quick Overview for HNRNPM antibody (N-Term) (ABIN2778962)

Target: HNRNPM (Heterogeneous Nuclear Ribonucleoprotein M (HNRNPM))

Reactivity: Human, Mouse, Rat, Cow, Pig, Dog, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNRNPM antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HNRNPM Antibody

Binding Specificity: N-Term

Sequence: ATEIKMEEES GAPGVPSGNG APGPKGEGER PAQNEKRKEK NIKRGGNRFE

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 93%, Horse: 92%, Human: 100%, Mouse: 92%, Pig: 100%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against HNRPM. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HNRPM

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 691 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-HNRNPM antibody (ABIN2778962)

Huelga, Vu, Arnold, Liang, Liu, Yan, Donohue, Shiue, Hoon, Brenner, Ares, Yeo: "Integrative genome-wide analysis reveals cooperative regulation of alternative splicing by hnRNP proteins." in: Cell reports, Vol. 1, Issue 2, pp. 167-78, (2012) (PubMed).

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for HNRNPM

Target: HNRNPM (Heterogeneous Nuclear Ribonucleoprotein M (HNRNPM))

Alternative Name: HNRPM

Background: HNRPM belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. HNRPM has three repeats of quasi-RRM domains that bind to RNAs. HNRPM also constitutes a monomer of the N-acetylglucosamine-specific receptor which is postulated to trigger selective recycling of immature GlcNAc-bearing thyroglobulin molecules.This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs. This protein also constitutes a monomer of the N-acetylglucosamine-specific receptor which is postulated to trigger selective recycling of immature GlcNAc-bearing thyroglobulin molecules. Multiple alternatively spliced transcript variants are known for this gene but only two transcripts has been isolated.
Alias Symbols: DKFZp547H118, HNRNPM, HNRNPM4, HNRPM4, HTGR1, NAGR1, CEAR, HNRPM, hnRNP M
Protein Interaction Partner: UBC, TP53, AKAP9, TEKT4, GMCL1P1, LMO2, HNRNPF, SUMO2, SUMO3, CEP250, MDM2, HAUS1, CDC37, IVNS1ABP, STAU1, SUMO1, RPA3, RPA2, RPA1, EED, rev, RTCB, RPL26L1, RFC4, PSMB8, NMT1, MRE11A, FLII, IGF2BP3, EIF2B2, EIF2B3, RPL27, RPL19, DHX9, DDX1, ABCF1, PARK2,
Protein Size: 691

Molecular Weight: 73 kDa

Gene ID: 4670

NCBI Accession: NM_031203, NP_112480

UniProt: P52272