PCBP1 antibody (Middle Region)

Catalog No. ABIN2778970

This Rabbit Polyclonal antibody specifically detects PCBP1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Rabbit, Dog, Guinea Pig and Horse.

Quick Overview for PCBP1 antibody (Middle Region) (ABIN2778970)

Target: PCBP1 (Poly(rC) Binding Protein 1 (PCBP1))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCBP1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PCBP1 Antibody

Binding Specificity: Middle Region

Sequence: CSDAVGYPHA THDLEGPPLD AYSIQGQHTI SPLDLAKLNQ VARQQSHFAM

Predicted Reactivity: Cow: 93%, Dog: 92%, Guinea Pig: 93%, Horse: 93%, Human: 93%, Mouse: 93%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against PCBP1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PCBP1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 356 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PCBP1

Target: PCBP1 (Poly(rC) Binding Protein 1 (PCBP1))

Alternative Name: PCBP1

Background: PCBP1 appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. PCBP1 is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.This intronless gene is thought to be generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 has paralogues PCBP3 and PCBP4 which is thought to arose as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding proteins. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.
Alias Symbols: HNRPX, HNRPE1, hnRNP-X, hnRNP-E1
Protein Interaction Partner: HUWE1, UBC, SNRPA, TP53, SUMO2, SUMO3, TUBGCP3, STAU1, ERG, EED, TUBB6, TUBB2A, PFKP, PFKM, SRPK2, SRPK1, SRPK3, FBXO6, TARDBP, UBD, ADRB2, NPM1, LMNA, HMGA1, CD81, PAN2, PRPF8, PPIG, PRPF3, UBL4A, ANP32A, VCAM1, NOS2, ITGA4, FN1, ATF2, ZNF830, ZCCHC10, P
Protein Size: 356

Molecular Weight: 39 kDa

Gene ID: 5093

NCBI Accession: NM_006196, NP_006187

UniProt: Q15365