PCBP1 antibody (Middle Region)

Catalog No. ABIN2778971

This anti-PCBP1 antibody is a Rabbit Polyclonal antibody detecting PCBP1 in WB and IHC. Suitable for Human, Mouse, Rat, Cow, Rabbit, Dog, Guinea Pig and Horse. This Primary Antibody has been cited in 1 publication.

Quick Overview for PCBP1 antibody (Middle Region) (ABIN2778971)

Target: PCBP1 (Poly(rC) Binding Protein 1 (PCBP1))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PCBP1 Antibody

Binding Specificity: Middle Region

Sequence: PHATHDLEGP PLDAYSIQGQ HTISPLDLAK LNQVARQQSH FAMMHGGTGF

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against PCBP1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Human PCBP1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 356 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-PCBP1 antibody (ABIN2778971)

Shi, Bencze, Stemmler, Philpott: "A cytosolic iron chaperone that delivers iron to ferritin." in: Science (New York, N.Y.), Vol. 320, Issue 5880, pp. 1207-10, (2008) (PubMed).

Target Details for PCBP1

Target: PCBP1 (Poly(rC) Binding Protein 1 (PCBP1))

Alternative Name: PCBP1

Background: PCBP1 appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. PCBP1 is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.This intronless gene is thought to be generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 has paralogues PCBP3 and PCBP4 which is thought to arose as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding proteins. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.
Alias Symbols: HNRPE1, HNRPX, hnRNP-E1, hnRNP-X
Protein Interaction Partner: HUWE1, UBC, SNRPA, TP53, SUMO2, SUMO3, TUBGCP3, STAU1, ERG, EED, TUBB6, TUBB2A, PFKP, PFKM, SRPK2, SRPK1, SRPK3, FBXO6, TARDBP, UBD, ADRB2, NPM1, LMNA, HMGA1, CD81, PAN2, PRPF8, PPIG, PRPF3, UBL4A, ANP32A, VCAM1, NOS2, ITGA4, FN1, ATF2, ZNF830, ZCCHC10, P
Protein Size: 356

Molecular Weight: 37 kDa

Gene ID: 5093

NCBI Accession: NM_006196, NP_006187

UniProt: Q15365