RG9MTD1 antibody (Middle Region)

Catalog No. ABIN2779118

The Rabbit Polyclonal anti-RG9MTD1 antibody has been validated for WB. It is suitable to detect RG9MTD1 in samples from Human, Rabbit, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig and Horse. There is 1 publication available.

Quick Overview for RG9MTD1 antibody (Middle Region) (ABIN2779118)

Target: RG9MTD1 (RNA (Guanine-9-) Methyltransferase Domain Containing 1 (RG9MTD1))

Reactivity: Human, Rabbit, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RG9MTD1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RG9MTD1 Antibody

Binding Specificity: Middle Region

Sequence: MKRKELQNTV SQLLESEGWN RRNVDPFHIY FCNLKIDGAL HRELVKRYQE

Predicted Reactivity: Cow: 86%, Dog: 79%, Guinea Pig: 92%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 86%, Rat: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against RG9MTD1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RG9MTD1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 403 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-RG9MTD1 antibody (ABIN2779118)

Szczepanowska, Maiti, Kukat, Hofsetz, Nolte, Senft, Becker, Ruzzenente, Hornig-Do, Wibom, Wiesner, Krüger, Trifunovic: "CLPP coordinates mitoribosomal assembly through the regulation of ERAL1 levels." in: The EMBO journal, Vol. 35, Issue 23, pp. 2566-2583, (2016) (PubMed).

Target Details for RG9MTD1

Target: RG9MTD1 (RNA (Guanine-9-) Methyltransferase Domain Containing 1 (RG9MTD1))

Alternative Name: RG9MTD1

Background: RG9MTD1 functions in mitochondrial tRNA maturation. Part of mitochondrial ribonuclease P, an enzyme composed of MRPP1/RG9MTD1, MRPP2/HSD17B10 and MRPP3/KIAA0391, which cleaves tRNA molecules in their 5'-ends.
Alias Symbols: FLJ20432, MRPP1, RG9MTD1
Protein Interaction Partner: CEP76, CEP57, TP53, SUMO2, SUMO3, GRSF1, UBC, ASB1, UBD, CAND1, CUL3, HDGF, USP39,
Protein Size: 403

Molecular Weight: 47 kDa

Gene ID: 54931

NCBI Accession: NM_017819, NP_060289

UniProt: Q7L0Y3