DAZAP1 antibody (C-Term)

Catalog No. ABIN2779143

This Rabbit Polyclonal antibody specifically detects DAZAP1 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Dog, Guinea Pig, Zebrafish (Danio rerio) and Horse.

Quick Overview for DAZAP1 antibody (C-Term) (ABIN2779143)

Target: DAZAP1 (DAZ Associated Protein 1 (DAZAP1))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Zebrafish (Danio rerio), Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAZAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-DAZAP1 Antibody

Binding Specificity: C-Term

Sequence: LAFPPPPSQA APDMSKPPTA QPDFPYGQYA GYGQDLSGFG QGFSDPSQQP

Predicted Reactivity: Dog: 86%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 100%, Rat: 93%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against DAZAP1. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human DAZAP1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 407 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for DAZAP1

Target: DAZAP1 (DAZ Associated Protein 1 (DAZAP1))

Alternative Name: DAZAP1

Background: In mammals, the Y chromosome directs the development of the testes and plays an important role in spermatogenesis. A high percentage of infertile men have deletions that map to regions of the Y chromosome. The DAZ (deleted in azoospermia) gene cluster maps to the AZFc region of the Y chromosome and is deleted in many azoospermic and severely oligospermic men. It is thought that the DAZ gene cluster arose from the transposition, amplification, and pruning of the ancestral autosomal gene DAZL also involved in germ cell development and gametogenesis. DAZAP1 is a RNA-binding protein with two RNP motifs that was originally identified by its interaction with the infertility factors DAZ and DAZL.In mammals, the Y chromosome directs the development of the testes and plays an important role in spermatogenesis. A high percentage of infertile men have deletions that map to regions of the Y chromosome. The DAZ (deleted in azoospermia) gene cluster maps to the AZFc region of the Y chromosome and is deleted in many azoospermic and severely oligospermic men. It is thought that the DAZ gene cluster arose from the transposition, amplification, and pruning of the ancestral autosomal gene DAZL also involved in germ cell development and gametogenesis. This gene encodes a RNA-binding protein with two RNP motifs that was originally identified by its interaction with the infertility factors DAZ and DAZL. Two isoforms are encoded by transcript variants of this gene.In mammals, the Y chromosome directs the development of the testes and plays an important role in spermatogenesis. A high percentage of infertile men have deletions that map to regions of the Y chromosome. The DAZ (deleted in azoospermia) gene cluster maps to the AZFc region of the Y chromosome and is deleted in many azoospermic and severely oligospermic men. It is thought that the DAZ gene cluster arose from the transposition, amplification, and pruning of the ancestral autosomal gene DAZL also involved in germ cell development and gametogenesis. This gene encodes a RNA-binding protein with two RNP motifs that was originally identified by its interaction with the infertility factors DAZ and DAZL. Two isoforms are encoded by transcript variants of this gene.
Protein Interaction Partner: UBC, WWOX, RPA3, RPA2, RPA1, SUZ12, RNF2, EZH2, BMI1, rev, ITCH, RAD52, VCAM1, ITGA4, FN1, BRCA1, CAND1, COPS5, CUL1, CUL2, CUL3, CUL4B, CUL5, NEDD8, ELAVL1, SUMO2, DAZ1, DAZL,
Protein Size: 407

Molecular Weight: 45 kDa

Gene ID: 26528

NCBI Accession: NM_018959, NP_061832

UniProt: Q96EP5