Calreticulin antibody (C-Term)

Catalog No. ABIN2779285

This Rabbit Polyclonal antibody specifically detects Calreticulin in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Guinea Pig, Dog, Rabbit, Horse, Zebrafish (Danio rerio), Saccharomyces cerevisiae and Goatand has been mentioned in 1 publication.

Quick Overview for Calreticulin antibody (C-Term) (ABIN2779285)

Target: Calreticulin (CALR)

Reactivity: Human, Mouse, Rat, Guinea Pig, Dog, Rabbit, Horse, Zebrafish (Danio rerio), Saccharomyces cerevisiae, Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calreticulin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Calreticulin Antibody

Binding Specificity: C-Term

Sequence: FGNETWGVTK AAEKQMKDKQ DEEQRLKEEE EDKKRKEEEE AEDKEDDEDK

Predicted Reactivity: Dog: 100%, Goat: 86%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Yeast: 92%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against CALR. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human CALR

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 417 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Calreticulin antibody (ABIN2779285)

Taguchi, Rho, Yan, Zhang, Zhao, Xu, Tripathi, Wang, Brenner, Kucherlapati, Kucherlapati, Boutin, Wang, DePinho, Feng, Lampe, Hanash: "MAPRE1 as a plasma biomarker for early-stage colorectal cancer and adenomas." in: Cancer prevention research (Philadelphia, Pa.), Vol. 8, Issue 11, pp. 1112-9, (2015) (PubMed).

Target Details for Calreticulin

Target: Calreticulin (CALR)

Alternative Name: CALR

Background: Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium.
Alias Symbols: RO, CRT, SSA, cC1qR
Protein Interaction Partner: HUWE1, UBC, LGR4, MDM2, RNF2, SUZ12, rev, AARSD1, THG1L, NSUN2, SH3GLB1, EEF2K, ARPC1A, ANP32B, ACTR2, ARPC1B, ARPC3, ARPC5, TPD52L2, SH3GL1, RPA3, RPA1, PRKACA, PFDN5, LPP, PARK2, UPF2, UBD, SOX2, PDIA3, CLN5, ITGA3, HLA-E, HLA-A, CALR, SLC27A2, env, MTN
Protein Size: 417

Molecular Weight: 48 kDa

Gene ID: 811

NCBI Accession: NM_004343, NP_004334

UniProt: P27797

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response