NK2 Homeobox 5 antibody (N-Term)

Catalog No. ABIN2779388

This Rabbit Polyclonal antibody specifically detects NK2 Homeobox 5 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit and Horse.

Quick Overview for NK2 Homeobox 5 antibody (N-Term) (ABIN2779388)

Target: NK2 Homeobox 5 (NKX2-5)

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NK2 Homeobox 5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NK2 Homeobox 5 Antibody

Binding Specificity: N-Term

Sequence: ELGRAPSPAK CASAFPAAPA FYPRAYSDPD PAKDPRAEKK ELCALQKAVE

Predicted Reactivity: Cow: 85%, Dog: 85%, Guinea Pig: 92%, Horse: 77%, Human: 100%, Mouse: 92%, Rabbit: 77%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against NKX2-5. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the n terminal region of human Nkx2-5

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 324 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NK2 Homeobox 5

Target: NK2 Homeobox 5 (NKX2-5)

Alternative Name: Nkx2-5

Background: Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development. It has been demonstrated that a Drosophila homeobox-containing gene called 'tinman' is expressed in the developing dorsal vessel and in the equivalent of the vertebrate heart. Mutations in tinman result in loss of heart formation in the embryo, suggesting that tinman is essential for Drosophila heart formation. Furthermore, abundant expression of Csx, the presumptive mouse homolog of tinman, is observed only in the heart from the time of cardiac differentiation. CSX, the human homolog of murine Csx, has a homeodomain sequence identical to that of Csx and is expressed only in the heart, again suggesting that CSX plays an important role in human heart formation.Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development (Shiojima et al., 1995 [PubMed 7665173]). It has been demonstrated that a Drosophila homeobox-containing gene called 'tinman' is expressed in the developing dorsal vessel and in the equivalent of the vertebrate heart. Mutations in tinman result in loss of heart formation in the embryo, suggesting that tinman is essential for Drosophila heart formation. Furthermore, abundant expression of Csx, the presumptive mouse homolog of tinman, is observed only in the heart from the time of cardiac differentiation. CSX, the human homolog of murine Csx, has a homeodomain sequence identical to that of Csx and is expressed only in the heart, again suggesting that CSX plays an important role in human heart formation.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-1211 U34962.1 1-1211 1212-1585 BC025711.1 1143-1516
Alias Symbols: CSX, CSX1, NKX2.5, NKX2E, NKX4-1, VSD3, CHNG5, HLHS2
Protein Interaction Partner: JARID2, GATA4, KDM6A, SMAD4, CAMTA2, TLE1, HDAC1, ID3, ID2, ID1, HIPK1, HIPK2, FOXH1, TBX5, TBX2, SRF, CSNK2A1,
Protein Size: 324

Molecular Weight: 35 kDa

Gene ID: 1482

NCBI Accession: NM_004387, NP_004378

UniProt: P52952

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development