EGR1 antibody (N-Term)

Catalog No. ABIN2779517

This Rabbit Polyclonal antibody specifically detects EGR1 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Horse, Cow, Rabbit and Pig and has been mentioned in 7+ publications.

Quick Overview for EGR1 antibody (N-Term) (ABIN2779517)

Target: EGR1 (Early Growth Response 1 (EGR1))

Reactivity: Human, Mouse, Rat, Horse, Cow, Rabbit, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-EGR1 Antibody

Binding Specificity: N-Term

Sequence: DNYPKLEEMM LLSNGAPQFL GAAGAPEGSG SNSSSSSSGG GGGGGGGSNS

Predicted Reactivity: Cow: 87%, Horse: 93%, Human: 100%, Mouse: 86%, Pig: 100%, Rabbit: 86%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against EGR1. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human EGR1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 543 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-EGR1 antibody (ABIN2779517)

Balakrishnan, Stykel, Touahri, Stratton, Biernaskie, Schuurmans: "Temporal Analysis of Gene Expression in the Murine Schwann Cell Lineage and the Acutely Injured Postnatal Nerve." in: PLoS ONE, Vol. 11, Issue 4, pp. e0153256, (2016) (PubMed).

Ohata, Yamazaki, Kawai, Tsugawa, Tachikawa, Koinuma, Miyagawa, Kimoto, Nakayama, Namba, Yamamoto, Okano, Ozono, Michigami: "Elevated fibroblast growth factor 23 exerts its effects on placenta and regulates vitamin D metabolism in pregnancy of Hyp mice." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 29, Issue 7, pp. 1627-38, (2014) (PubMed).

Lane, Yu, Lackey, Chen, Marzluff, Cook: "Cell cycle-regulated protein abundance changes in synchronously proliferating HeLa cells include regulation of pre-mRNA splicing proteins." in: PLoS ONE, Vol. 8, Issue 3, pp. e58456, (2013) (PubMed).

Revest, Kaouane, Mondin, Le Roux, Rougé-Pont, Vallée, Barik, Tronche, Desmedt, Piazza: "The enhancement of stress-related memory by glucocorticoids depends on synapsin-Ia/Ib." in: Molecular psychiatry, Vol. 15, Issue 12, pp. 1125, 1140-51, (2010) (PubMed).

Jaluria, Konstantopoulos, Betenbaugh, Shiloach: "Egr1 and Gas6 facilitate the adaptation of HEK-293 cells to serum-free media by conferring enhanced viability and higher growth rates." in: Biotechnology and bioengineering, Vol. 99, Issue 6, pp. 1443-52, (2008) (PubMed).

Wenzel, Daskalow, Herse, Seitz, Zacharias, Schenk, Schulz, Hubner, Micheel, Schlag, Osterziel, Ozcelik, Scherneck, Jandrig: "Expression of the protein phosphatase 1 inhibitor KEPI is downregulated in breast cancer cell lines and tissues and involved in the regulation of the tumor suppressor EGR1 via the MEK-ERK pathway." in: Biological chemistry, Vol. 388, Issue 5, pp. 489-95, (2007) (PubMed).

Barbolina, Adley, Ariztia, Liu, Stack: "Microenvironmental regulation of membrane type 1 matrix metalloproteinase activity in ovarian carcinoma cells via collagen-induced EGR1 expression." in: The Journal of biological chemistry, Vol. 282, Issue 7, pp. 4924-31, (2007) (PubMed).

Target Details for EGR1

Target: EGR1 (Early Growth Response 1 (EGR1))

Alternative Name: EGR1

Background: Early Growth Response Protein 1 (EGR1, Krox-24 protein, nerve growth factor-induced protein A, Transcription factor ETR103, Zinc finger protein 225) belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene. The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: AT225, G0S30, KROX-24, NGFI-A, TIS8, ZIF-268, ZNF225
Protein Interaction Partner: CEBPB, WT1, SUMO1, TP53, MDM2, EP300, CDKN2A, SRA1, IL1B, PTEN, SP1, SNAI1, TBX2, PFDN5, SREBF2, ERBB3, NAB2, PITX1, NAB1, NFATC2, CREBBP, PSMA3, JUN, RELA, CSNK2A1, NFATC1, GADD45B, GADD45A,
Protein Size: 543

Molecular Weight: 57 kDa

Gene ID: 1958

NCBI Accession: NM_001964, NP_001955

UniProt: P18146

Pathways: Regulation of Carbohydrate Metabolic Process, Regulation of long-term Neuronal Synaptic Plasticity