EPAS1 antibody (Middle Region)

Catalog No. ABIN2779523

The Rabbit Polyclonal anti-EPAS1 antibody has been validated for WB. It is suitable to detect EPAS1 in samples from Human, Rat, Mouse, Horse, Cow, Guinea Pig, Rabbit, Dog and Zebrafish (Danio rerio).

Quick Overview for EPAS1 antibody (Middle Region) (ABIN2779523)

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Reactivity: Human, Rat, Mouse, Horse, Cow, Guinea Pig, Rabbit, Dog, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPAS1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EPAS1 Antibody

Binding Specificity: Middle Region

Sequence: ESLFKPHLMA MNSIFDSSGK GAVSEKSNFL FTKLKEEPEE LAQLAPTPGD

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against EPAS1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human EPAS1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 870 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for EPAS1

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Alternative Name: EPAS1

Background: EPAS1 is a transcription factor involved in the induction of oxygen regulated genes. EPAS1 binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. EPAS1 regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. EPAS1 may also play a role in the formation of the endothelium that gives rise to the blood brain barrier. EPAS1 is a potent activator of the Tie-2 tyrosine kinase expression. The activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction of EPAS1 with redox regulatory protein APEX seems to activate CTAD.
Alias Symbols: HIF2A, HLF, MOP2, PASD2, ECYT4, bHLHe73
Protein Interaction Partner: MAP1LC3A, SQSTM1, LAMP1, BATF2, BATF3, BATF, DDIT3, DBP, NDN, JUN, EPAS1, NPM1, BBS4, BBS2, BBS1, Arnt, VHL, STAT5A, SMAD3, EWSR1, MED11, MED8, EGLN3, PSMD1, PSMB1, MED1, PHB, MITF, HIF1A, EP300, EMD, CTNNB1, CREBBP, EGLN2, DPY30, MED10, MED25, ARID1B, WR
Protein Size: 870

Molecular Weight: 90 kDa

Gene ID: 2034

NCBI Accession: NM_001430, NP_001421

UniProt: Q99814

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect