ETV4 antibody (Middle Region)

Catalog No. ABIN2779533

This Rabbit Polyclonal antibody specifically detects ETV4 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Pig, Horse and Rabbit and has been mentioned in 5+ publications.

Quick Overview for ETV4 antibody (Middle Region) (ABIN2779533)

Target: ETV4 (Ets Variant 4 (ETV4))

Reactivity: Human, Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ETV4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ETV4 Antibody

Binding Specificity: Middle Region

Sequence: HSSVFQQPLD ICHSFTSQGG GREPLPAPYQ HQLSEPCPPY PQQSFKQEYH

Predicted Reactivity: Cow: 100%, Dog: 86%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against ETV4. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ETV4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 484 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ETV4 antibody (ABIN2779533)

Kedage, Selvaraj, Nicholas, Budka, Plotnik, Jerde, Hollenhorst: "An Interaction with Ewing's Sarcoma Breakpoint Protein EWS Defines a Specific Oncogenic Mechanism of ETS Factors Rearranged in Prostate Cancer." in: Cell reports, Vol. 17, Issue 5, pp. 1289-1301, (2016) (PubMed).

Okimoto, Breitenbuecher, Olivas, Wu, Gini, Hofree, Asthana, Hrustanovic, Flanagan, Tulpule, Blakely, Haringsma, Simmons, Gowen, Suh, Miller, Ali, Schuler, Bivona: "Inactivation of Capicua drives cancer metastasis." in: Nature genetics, Vol. 49, Issue 1, pp. 87-96, (2016) (PubMed).

Selvaraj, Budka, Ferris, Jerde, Hollenhorst: "Prostate cancer ETS rearrangements switch a cell migration gene expression program from RAS/ERK to PI3K/AKT regulation." in: Molecular cancer, Vol. 13, pp. 61, (2014) (PubMed).

Hollenhorst, Paul, Ferris, Graves: "The ETS gene ETV4 is required for anchorage-independent growth and a cell proliferation gene expression program in PC3 prostate cells." in: Genes & cancer, Vol. 1, Issue 10, pp. 1044-1052, (2011) (PubMed).

Hollenhorst, Ferris, Hull, Chae, Kim, Graves: "Oncogenic ETS proteins mimic activated RAS/MAPK signaling in prostate cells." in: Genes & development, Vol. 25, Issue 20, pp. 2147-57, (2011) (PubMed).

Target Details for ETV4

Target: ETV4 (Ets Variant 4 (ETV4))

Alternative Name: ETV4

Background: The protein encoded by the ETV4 gene is known to play a role in ovarian and breast malignancies as well as in the early stage of colorectal carcinogenesis.
Alias Symbols: E1A-F, E1AF, PEA3, PEAS3
Protein Interaction Partner: STK11, RFWD2, TP63, ATXN1, HTT, APP, UBC, SMAD2, SUMO2, SUMO1, SAE1, UBA2, HOXD4, EP300, SRF,
Protein Size: 484

Molecular Weight: 54 kDa

Gene ID: 2118

NCBI Accession: NM_001986, NP_001977

UniProt: P43268