SIRT4 antibody (Middle Region)

Catalog No. ABIN2779654

This Rabbit Polyclonal antibody specifically detects SIRT4 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Pig, Horse, Rabbit, Sheep and Guinea Pig.

Quick Overview for SIRT4 antibody (Middle Region) (ABIN2779654)

Target: SIRT4 (Sirtuin 4 (SIRT4))

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig, Horse, Rabbit, Sheep, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SIRT4 Antibody

Binding Specificity: Middle Region

Sequence: QVPTCVQCGG HLKPDVVFFG DTVNPDKVDF VHKRVKEADS LLVVGSSLQV

Predicted Reactivity: Cow: 93%, Dog: 79%, Guinea Pig: 79%, Horse: 79%, Human: 100%, Mouse: 93%, Pig: 86%, Rabbit: 93%, Rat: 93%, Sheep: 92%

Characteristics: This is a rabbit polyclonal antibody against SIRT4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SIRT4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 314 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SIRT4

Target: SIRT4 (Sirtuin 4 (SIRT4))

Alternative Name: SIRT4

Background: SIRT4 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined, however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. SIRT4 is included in class IV of the sirtuin family.This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined, however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.
Alias Symbols: MGC130046, MGC130047, MGC57437, SIR2L4, sirtuin 4
Protein Interaction Partner: SKP2, Glud1, UBC, SIRT3, IDE, SLC25A6, SLC25A5,
Protein Size: 314

Molecular Weight: 35 kDa

Gene ID: 23409

NCBI Accession: NM_012240, NP_036372

UniProt: Q9Y6E7

Pathways: Negative Regulation of Hormone Secretion