HCLS1 antibody (N-Term)

Catalog No. ABIN2779751

Product Details anti-HCLS1 Antibody

Target: HCLS1 (Hematopoietic Cell-Specific Lyn Substrate 1 (HCLS1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig, Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HCLS1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: FGVERDRMDK SAVGHEYVAE VEKHSSQTDA AKGFGGKYGV ERDRADKSAV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 92%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against HCLS1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HCLS1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 486 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-HCLS1 antibody (ABIN2779751)

Ruzzene, Penzo, Pinna: "Protein kinase CK2 inhibitor 4,5,6,7-tetrabromobenzotriazole (TBB) induces apoptosis and caspase-dependent degradation of haematopoietic lineage cell-specific protein 1 (HS1) in Jurkat cells." in: The Biochemical journal, Vol. 364, Issue Pt 1, pp. 41-7, (2002) (PubMed).

Target Details for HCLS1

Target: HCLS1 (Hematopoietic Cell-Specific Lyn Substrate 1 (HCLS1))

Alternative Name: HCLS1 (HCLS1 Products)

Background: HS1 which is hematopoietic lineage cell-specific protein 1, is a substrate of protein tyrosine kinases in lymphocytes, it binds to F-actin, and promotes Arp2/3 complex-mediated actin polymerization. However, the mechanism for the interaction between HS1 and F-actin has not yet been fully characterized. HS1 contains 3.5 tandem repeats, a coiled-coil region, and an SH3 domain at the C terminus. Unlike cortactin, which is closely related to HS1 and requires absolutely the repeat domain for F-actin binding, an HS1 mutant with deletion of the repeat domain maintains a significant F-actin binding activity. Deletion of the coiled-coil region abolished the ability of HS1 to bind to actin filaments and to activate the Arp2/3 complex for actin nucleation and actin branching.
Alias Symbols: HS1, CTTNL
Protein Interaction Partner: SH2D4A, UBC, Mbp, Dlg4, NOTCH1, QKI, TERF1, TRIM29, SSBP3, BLZF1, IKBKG, LZTR1, ZBTB25, HS1BP3, HAX1, ACTR2, MAP4K1, CASP3, SYK, WAS, LYN, FGR, CSNK2A1, CD79A, ACTA1, GRB2, ACTR3,
Protein Size: 486

Molecular Weight: 54 kDa

Gene ID: 3059

NCBI Accession: NM_005335, NP_005326

UniProt: P14317

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Maintenance of Protein Location