AIRE antibody (N-Term)

Catalog No. ABIN2779776

Product Details anti-AIRE Antibody

Target: AIRE (Autoimmune Regulator (AIRE))

Binding Specificity: N-Term

Reactivity: Human, Rat, Dog, Horse, Cow, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AIRE antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: DFWRVLFKDY NLERYGRLQP ILDSFPKDVD LSQPRKGRKP PAVPKALVPP

Predicted Reactivity: Cow: 79%, Dog: 92%, Horse: 86%, Human: 100%, Pig: 93%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against AIRE. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human AIRE

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 545 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-AIRE antibody (ABIN2779776)

Tao, Kupfer, Stewart, Williams-Skipp, Crowell, Patel, Sain, Scheinman: "AIRE recruits multiple transcriptional components to specific genomic regions through tethering to nuclear matrix." in: Molecular immunology, Vol. 43, Issue 4, pp. 335-45, (2005) (PubMed).

Target Details for AIRE

Target: AIRE (Autoimmune Regulator (AIRE))

Alternative Name: AIRE (AIRE Products)

Background: AIRE is a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CBP. At least three splice variant mRNAs products have been described including one which results in a premature stop codon and a transcript predicted to be a candidate for nuclear-mediated decay (NMD). Defects in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).
Alias Symbols: APS1, APSI, PGA1, AIRE1, APECED
Protein Interaction Partner: TUBB, RAVER1, TUBA1C, TMPRSS13, CCDC87, FAT1, GIGYF2, SRRM2, TUBB4B, EIF4EBP3, UBB, HSP90AB1, HSPA8, HSPA5, HSPA1L, HSPA1A, ATP4A, HIST3H3, HDAC2, HDAC1, DAXX, AIRE, UBAC1, HIST1H3A, HIST2H2BE, HSPA4, SRSF1, SMC1A, XPO1, TOP2A, SSRP1, SNRPD3, SNRPB, SRSF3
Protein Size: 545

Molecular Weight: 58 kDa

Gene ID: 326

NCBI Accession: NM_000383, NP_000374

UniProt: O43918

Pathways: Chromatin Binding