NFAT1 antibody (N-Term)

Catalog No. ABIN2779960

This anti-NFAT1 antibody is a Rabbit Polyclonal antibody detecting NFAT1 in WB. Suitable for Human, Mouse, Rat, Dog, Horse, Cow and Pig. This Primary Antibody has been cited in 2+ publications.

Quick Overview for NFAT1 antibody (N-Term) (ABIN2779960)

Target: NFAT1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1))

Reactivity: Human, Mouse, Rat, Dog, Horse, Cow, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFAT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NFAT1 Antibody

Binding Specificity: N-Term

Sequence: MDVPEPQPDP DGGDGPGHEP GGSPQDELDF SILFDYDYLN PIEEEPIAHK

Predicted Reactivity: Cow: 86%, Dog: 82%, Horse: 93%, Human: 93%, Mouse: 100%, Pig: 79%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against NFATC2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of mouse NFATC2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 927 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-NFAT1 antibody (ABIN2779960)

Nagamoto-Combs, Combs: "Microglial phenotype is regulated by activity of the transcription factor, NFAT (nuclear factor of activated T cells)." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 28, pp. 9641-6, (2010) (PubMed).

Heer, Clarke, Rigas, Cheek, Pickard, Leung: "Phenotypic modulation of human urinary tract stroma-derived fibroblasts by transforming growth factor beta3." in: Urology, Vol. 76, Issue 2, pp. 509.e13-20, (2010) (PubMed).

Target Details for NFAT1

Target: NFAT1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1))

Alternative Name: NFATC2

Background: NFATC2 is a member of The NF-AT family of potent transcription factors that are essential for T cell activation
Alias Symbols: NFAT1, Nfatp, NF-ATc2, NFAT1-D, AI607462
Protein Interaction Partner: Foxp3, Il4, Ifng, Nfatc2ip,
Protein Size: 927

Molecular Weight: 100 kDa

Gene ID: 18019

NCBI Accession: NM_010899, NP_035029

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, VEGF Signaling, BCR Signaling