MCAF1 antibody (Middle Region)

Catalog No. ABIN2780013

This anti-MCAF1 antibody is a Rabbit Polyclonal antibody detecting MCAF1 in WB. Suitable for Pig, Mouse and Rat.

Quick Overview for MCAF1 antibody (Middle Region) (ABIN2780013)

Target: MCAF1 (ATF7IP) (Activating Transcription Factor 7 Interacting Protein (ATF7IP))

Reactivity: Pig, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCAF1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MCAF1 Antibody

Binding Specificity: Middle Region

Sequence: MSTPEGEKSE KDGKAEEEER VPAEEQPPVR NEFSRRKRSK SEDMDSVESK

Predicted Reactivity: Mouse: 100%, Pig: 92%, Rat: 85%

Characteristics: This is a rabbit polyclonal antibody against Atf7ip. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Mouse Atf7ip

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1306 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MCAF1

Target: MCAF1 (ATF7IP) (Activating Transcription Factor 7 Interacting Protein (ATF7IP))

Alternative Name: Atf7ip

Background: Recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. Atf7ip can both act as an activator or a repressor depending on the context. It mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. Atf7ip is required to stimulate histone methyltransferase activity of SETDB1 and facilitate the conversion of dimethylated to trimethylated H3 'Lys-9' (H3K9me3). The complex formed with MBD1 and SETDB1 represses transcription and couples DNA methylation and histone H3 'Lys-9' trimethylation (H3K9me3). Atf7ip may have ATPase activity.
Alias Symbols: 2610204M12Rik, AM, Mcaf1
Protein Interaction Partner: Atf7, Gtf2f1, Gtf2e2, Aagab, Ccnh, Gtf2h1, Ercc3, Cdk7, C1qbp, POLR2H, POLR2G, POLR2D, POLR2C,
Protein Size: 1306

Molecular Weight: 138 kDa

Gene ID: 54343

NCBI Accession: NM_019426, NP_062299

UniProt: Q7TT18