DDX1 antibody (C-Term)

Catalog No. ABIN2780220

The Rabbit Polyclonal anti-DDX1 antibody has been validated for WB. It is suitable to detect DDX1 in samples from Human, Mouse, Rat, Dog, Rabbit, Horse, Cow, Guinea Pig and Zebrafish (Danio rerio).

Quick Overview for DDX1 antibody (C-Term) (ABIN2780220)

Target: DDX1 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 1 (DDX1))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Horse, Cow, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DDX1 Antibody

Binding Specificity: C-Term

Sequence: SQVEPDIKVP VDEFDGKVTY GQKRAAGGGS YKGHVDILAP TVQELAALEK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against DDX1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human DDX1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 740 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for DDX1

Target: DDX1 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 1 (DDX1))

Alternative Name: DDX1

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin.
Alias Symbols: DBP-RB, UKVH5d
Protein Interaction Partner: HUWE1, FUS, SUMO2, SUMO3, STAU1, UBC, SUMO1, NEDD8, Fbxl16, WWOX, ZBTB1, RPA3, RPA2, RPA1, rev, C14orf166, RTCB, RPL26L1, EIF3K, NELFB, EDC4, IGF2BP3, PDCD6, ABCF1, EIF2B2, EIF2B3, YBX3, RPL27, RFC4, RFC2, QARS, YBX1, NMT1, HNRNPM, MRE11A, KRT18, ILF2, HN
Protein Size: 740

Molecular Weight: 81 kDa

Gene ID: 1653

NCBI Accession: NM_004939, NP_004930

UniProt: Q92499

Pathways: Ribonucleoprotein Complex Subunit Organization