ERCC3 antibody (N-Term)

Catalog No. ABIN2780326

The Rabbit Polyclonal anti-ERCC3 antibody has been validated for WB. It is suitable to detect ERCC3 in samples from Human, Rat, Cow, Dog, Rabbit, Zebrafish (Danio rerio), Guinea Pig, Pig and Saccharomyces cerevisiae. There is 1 publication available.

Quick Overview for ERCC3 antibody (N-Term) (ABIN2780326)

Target: ERCC3 (DNA Repair Protein Complementing XP-B Cells (ERCC3))

Reactivity: Human, Rat, Cow, Dog, Rabbit, Zebrafish (Danio rerio), Guinea Pig, Pig, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERCC3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ERCC3 Antibody

Binding Specificity: N-Term

Sequence: MGKRDRADRD KKKSRKRHYE DEEDDEEDAP GNDPQEAVPS AAGKQVDESG

Predicted Reactivity: Cow: 86%, Dog: 86%, Guinea Pig: 79%, Human: 100%, Pig: 93%, Rabbit: 79%, Rat: 79%, Yeast: 90%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against ERCC3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ERCC3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 782 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ERCC3 antibody (ABIN2780326)

Vijai, Topka, Villano, Ravichandran, Maxwell, Maria, Thomas, Gaddam, Lincoln, Kazzaz, Wenz, Carmi, Schrader, Hart, Lipkin, Neuhausen, Walsh, Zhang, Lejbkowicz, Rennert, Stadler, Robson, Weitzel et al.: "A Recurrent ERCC3 Truncating Mutation Confers Moderate Risk for Breast Cancer. ..." in: Cancer discovery, Vol. 6, Issue 11, pp. 1267-1275, (2016) (PubMed).

Target Details for ERCC3

Target: ERCC3 (DNA Repair Protein Complementing XP-B Cells (ERCC3))

Alternative Name: ERCC3

Background: ERCC3 is an ATP-dependent DNA helicase that functions in nucleotide excision repair and complements xeroderma pigmentosum group B mutations. It also is the 89 kDa subunit of basal transcription factor 2 (TFIIH) and thus functions in class II transcription.
Alias Symbols: BTF2, GTF2H, RAD25, TFIIH, XPB
Protein Interaction Partner: XIAP, CEP70, UBC, rev, SRPK2, CDK7, TP53, E2F1, CDK8, CCNC, GTF2H5, ERCC2, MSANTD2, UVSSA, POLR2C, KPNA3, RAD52, MMS19, SIX5, GTF2H4, GTF2H3, GTF2H1, tat, COPS2, GTF2H2, NEDD8, ERCC6, POLR2A, AR, CCNH, MYC, MNAT1, ZSCAN1, ATF7IP, GCN1L1, ERCC5, BCR, PSMC5
Protein Size: 782

Molecular Weight: 89 kDa

Gene ID: 2071

NCBI Accession: NM_000122, NP_000113

UniProt: P19447

Pathways: DNA Damage Repair