WT1 antibody (Middle Region)

Catalog No. ABIN2780340

This Rabbit Polyclonal antibody specifically detects WT1 in IHC and WB. It exhibits reactivity toward Human, Rat, Mouse, Cow, Rabbit, Dog, Pig, Zebrafish (Danio rerio) and Horse.

Quick Overview for WT1 antibody (Middle Region) (ABIN2780340)

Target: WT1 (Wilms Tumor 1 (WT1))

Reactivity: Human, Rat, Mouse, Cow, Rabbit, Dog, Pig, Zebrafish (Danio rerio), Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WT1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Product Details anti-WT1 Antibody

Binding Specificity: Middle Region

Sequence: DHLKTHTRTH TGEKPFSCRW PSCQKKFARS DELVRHHNMH QRNMTKLQLA

Predicted Reactivity: Cow: 93%, Dog: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 93%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against WT1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human WT1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 514 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Alternative Name: WT1

Background: WT1 is a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a sm
Alias Symbols: GUD, WAGR, WIT-2, WT33, AWT1, NPHS4, EWS-WT1
Protein Interaction Partner: KRTAP10-3, KRTAP10-8, KRT40, EGR1, MDM2, DVL3, CIAO1, TP53, HSPA4, TAOK1, NPM3, ZNF205, SUZ12, MEN1, EZH2, DNMT1, WTIP, WTAP, PAWR, UBE2I, TP63, FHL2, TP73, PRKACA, CREBBP, U2AF2, PAX2, AREG,
Protein Size: 514

Molecular Weight: 56 kDa

Gene ID: 7490

NCBI Accession: NM_024424, NP_077742

UniProt: P19544

Pathways: Tube Formation