BTF3 antibody (Middle Region)

Catalog No. ABIN2780354

The Rabbit Polyclonal anti-BTF3 antibody has been validated for WB. It is suitable to detect BTF3 in samples from Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Cow, Guinea Pig, Horse, Rabbit and Saccharomyces cerevisiae. There is 1 publication available.

Quick Overview for BTF3 antibody (Middle Region) (ABIN2780354)

Target: BTF3 (Basic Transcription Factor 3 (BTF3))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Cow, Guinea Pig, Horse, Rabbit, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BTF3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BTF3 Antibody

Binding Specificity: Middle Region

Sequence: DSLTSLRRLA EALPKQSVDG KAPLATGEDD DDEVPDLVEN FDEASKNEAN

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 92%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against BTF3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human BTF3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 206 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-BTF3 antibody (ABIN2780354)

Thakur, Saxena, Singh, Haq, Katti, Singh, Tripathi: "Human beta casein fragment (54-59) modulates M. bovis BCG survival and basic transcription factor 3 (BTF3) expression in THP-1 cell line." in: PLoS ONE, Vol. 7, Issue 9, pp. e45905, (2012) (PubMed).

Target Details for BTF3

Target: BTF3 (Basic Transcription Factor 3 (BTF3))

Alternative Name: BTF3

Background: BTF3 forms a stable complex with RNA polymerase IIB and is required for transcriptional initiation. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene has multiple pseudogenes.
Alias Symbols: NACB, BTF3a, BTF3b, BETA-NAC
Protein Interaction Partner: TXLNA, TXLNB, NACA, UBC, PDCD4, PPP2R1A, gag-pol, ESR1, CTNNB1, NACA2, RWDD4, PDRG1, RPL29, RPL11, APP, H2AFX, USP17L9P, POLR2B, MED21, CSNK2B,
Protein Size: 206

Molecular Weight: 23 kDa

Gene ID: 689

NCBI Accession: NM_001037637, NP_001032726

UniProt: P20290