SPIB antibody (Middle Region)

Catalog No. ABIN2780475

This Rabbit Polyclonal antibody specifically detects SPIB in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse and Rabbitand has been mentioned in 1 publication.

Quick Overview for SPIB antibody (Middle Region) (ABIN2780475)

Target: SPIB (Spi-B Transcription Factor (Spi-1/PU.1 Related) (SPIB))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPIB antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SPIB Antibody

Binding Specificity: Middle Region

Sequence: WGQQKGNRKR MTYQKLARAL RNYAKTGEIR KVKRKLTYQF DSALLPAVRR

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against SPIB. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SPIB

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 262 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-SPIB antibody (ABIN2780475)

Zhao, Yao, Xu, Lambeth, Smith, Kgosana, Wang, Nair: "A functional MicroRNA-155 ortholog encoded by the oncogenic Marek's disease virus." in: Journal of virology, Vol. 83, Issue 1, pp. 489-92, (2008) (PubMed).

Target Details for SPIB

Target: SPIB (Spi-B Transcription Factor (Spi-1/PU.1 Related) (SPIB))

Alternative Name: SPIB

Background: SPI1 (MIM 165170) and SPIB are members of a subfamily of ETS (see ETS1, MIM 164720) transcription factors. ETS proteins share a conserved ETS domain that mediates specific DNA binding. SPIB and SPI1 bind to a purine-rich sequence, the PU box (5-prime-GAGG
Alias Symbols: SPI-B
Protein Interaction Partner: TMEM37, SSB, GATA1, IRF4, MAPK3, MAPK8, CREBBP, TBP, CEBPB, RB1, SPI1, JUN, CSNK2A2, CSNK2A1, SPIB, E2F4, E2F3, E2F2, E2F1,
Protein Size: 262

Molecular Weight: 29 kDa

Gene ID: 6689

NCBI Accession: NM_003121, NP_003112

UniProt: Q01892