TIMELESS antibody (N-Term)

Catalog No. ABIN2780561

The Rabbit Polyclonal anti-TIMELESS antibody has been validated for WB. It is suitable to detect TIMELESS in samples from Human, Mouse, Rat, Dog, Cow, Horse, Guinea Pig, Sheep, Pig, Rabbit and Zebrafish (Danio rerio).

Quick Overview for TIMELESS antibody (N-Term) (ABIN2780561)

Target: TIMELESS (Timeless Homolog (TIMELESS))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Guinea Pig, Sheep, Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TIMELESS antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TIMELESS Antibody

Binding Specificity: N-Term

Sequence: MDLHMMNCEL LATCSALGYL EGDTYHKEPD CLESVKDLIR YLRHEDETRD

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 100%, Rat: 93%, Sheep: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against TIMELESS. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TIMELESS

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1208 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for TIMELESS

Target: TIMELESS (Timeless Homolog (TIMELESS))

Alternative Name: TIMELESS

Background: The human Timeless protein interacts with both the circadian clock protein cryptochrome 2 and with the cell cycle checkpoint proteins Chk1 and the ATR-ATRIP complex and plays an important role in the DNA damage checkpoint response. Down-regulation of Timeless in human cells seriously compromises replication and intra-S checkpoints, indicating an intimate connection between the circadian cycle and the DNA damage checkpoints that is in part mediated by the Timeless protein.
Alias Symbols: TIM, TIM1, hTIM
Protein Interaction Partner: UBC, TUT1, TF, XRCC1, FKBP3, SP1, SUMO2, Tipin, Timeless, ZSCAN1, PCBD1, CRY1, PER2, PER1, CLSPN, PCNA, per, Clock, ATRIP, CRY2, CHEK1,
Protein Size: 1208

Molecular Weight: 139 kDa

Gene ID: 8914

NCBI Accession: NM_003920, NP_003911

UniProt: Q9UNS1

Pathways: Protein targeting to Nucleus, Photoperiodism