SREBF1 antibody (N-Term)

Catalog No. ABIN2780569

This anti-SREBF1 antibody is a Rabbit Polyclonal antibody detecting SREBF1 in WB. Suitable for Human, Rat, Mouse, Cow, Goat, Horse, Guinea Pig, Rabbit and Dog.

Quick Overview for SREBF1 antibody (N-Term) (ABIN2780569)

Target: SREBF1 (Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1))

Reactivity: Human, Rat, Mouse, Cow, Goat, Horse, Guinea Pig, Rabbit, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SREBF1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SREBF1 Antibody

Binding Specificity: N-Term

Sequence: AGRGRANGLD APRAGADRGA MDCTFEDMLQ LINNQDSDFP GLFDPPYAGS

Predicted Reactivity: Cow: 93%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against SREBF1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SREBF1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1177 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SREBF1

Target: SREBF1 (Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1))

Alternative Name: SREBF1

Background: SREBF1is a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17.This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Two transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: SREBP1, bHLHd1, SREBP-1c
Protein Interaction Partner: UBC, ERLIN2, FBXW7, SMARCD3, HSP90AA1, SUMO1, ATXN1L, INSIG2, SCAP, ATXN1, LMNA, INSIG1, EGF, SREBF2, SREBF1, ZBTB7A, ZBTB7C, SUMO2, MED6, MED13, MED12, MED24, MED26, MED17, MED23, MED14, USF1, MED1, GSK3B, CREBBP, CDK8, MED25, MED15, MED7, MED21, SIRT1,
Protein Size: 1177

Molecular Weight: 125 kDa

Gene ID: 6720

NCBI Accession: NM_001005291, NP_001005291

Pathways: AMPK Signaling, Caspase Cascade in Apoptosis, Negative Regulation of Hormone Secretion, Regulation of Lipid Metabolism by PPARalpha