KLF4 antibody (C-Term)

Catalog No. ABIN2780577

This Rabbit Polyclonal antibody specifically detects KLF4 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Cow, Horse, Dog, Rabbit, Guinea Pig and Zebrafish (Danio rerio)and has been mentioned in 1 publication.

Quick Overview for KLF4 antibody (C-Term) (ABIN2780577)

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Reactivity: Human, Mouse, Rat, Cow, Horse, Dog, Rabbit, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KLF4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-KLF4 Antibody

Binding Specificity: C-Term

Sequence: AGCGKTYTKS SHLKAHLRTH TGEKPYHCDW DGCGWKFARS DELTRHYRKH

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against KLF4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human KLF4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 470 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-KLF4 antibody (ABIN2780577)

Natesampillai, Kerkvliet, Leung, Veldhuis: "Regulation of Kruppel-like factor 4, 9, and 13 genes and the steroidogenic genes LDLR, StAR, and CYP11A in ovarian granulosa cells." in: American journal of physiology. Endocrinology and metabolism, Vol. 294, Issue 2, pp. E385-91, (2008) (PubMed).

Target Details for KLF4

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Alternative Name: KLF4

Background: The mammalian Kruppel-like transcription factor, KLF4 is involved in preventing centrosome amplification following DNA damage caused by gamma-irradiation. It is both necessary and sufficient in preventing centrosome amplification following gamma-radiation-induced DNA damage and does so by transcriptionally suppressing cyclin E expression n. Kruppel-like factor 4 is also known to exhibit checkpoint function during the G1/S and G2/M transitions of the cell cycle.
Alias Symbols: EZF, GKLF
Protein Interaction Partner: EP300, CREBBP, SETD7, CTBP1, Dlg4, APP, CUL2, VHL, UBC, TCEB2, SP1, RELA, PRKCD, PPARG, SMARCA4, HDAC2, HDAC7, KAT5, HDAC1, HDAC5, ELK1, KLF6, TP53,
Protein Size: 470

Molecular Weight: 50 kDa

Gene ID: 9314

NCBI Accession: NM_004235, NP_004226

UniProt: O43474

Pathways: Peptide Hormone Metabolism, Stem Cell Maintenance