RCAN1 antibody (Middle Region)

Catalog No. ABIN2780590

This Rabbit Polyclonal antibody specifically detects RCAN1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Sheep, Rabbit and Zebrafish (Danio rerio)and has been mentioned in 1 publication.

Quick Overview for RCAN1 antibody (Middle Region) (ABIN2780590)

Target: RCAN1 (Regulator of Calcineurin 1 (RCAN1))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Sheep, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RCAN1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RCAN1 Antibody

Binding Specificity: Middle Region

Sequence: PVINYDLLYA ISKLGPGEKY ELHAATDTTP SVVVHVCESD QEKEEEEEME

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Sheep: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against RCAN1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RCAN1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 252 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-RCAN1 antibody (ABIN2780590)

Voronov, Ochotny, Jaumouillé, Owen, Manolson, Aubin: "The R740S mutation in the V-ATPase a3 subunit increases lysosomal pH, impairs NFATc1 translocation, and decreases in vitro osteoclastogenesis." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 28, Issue 1, pp. 108-18, (2012) (PubMed).

Target Details for RCAN1

Target: RCAN1 (Regulator of Calcineurin 1 (RCAN1))

Alternative Name: RCAN1

Background: RCAN1 interacts with calcineurin A and inhibits calcineurin-dependent signaling pathways, possibly affecting central nervous system development. This gene is located in the minimal candidate region for the Down syndrome phenotype, and is overexpressed in the brain of Down syndrome fetuses. Chronic overexpression of this gene may lead to neurofibrillary tangles such as those associated with Alzheimer disease.The protein encoded by this gene interacts with calcineurin A and inhibits calcineurin-dependent signaling pathways, possibly affecting central nervous system development. This gene is located in the minimal candidate region for the Down syndrome phenotype, and is overexpressed in the brain of Down syndrome fetuses. Chronic overexpression of this gene may lead to neurofibrillary tangles such as those associated with Alzheimer disease. Three transcript variants encoding three different isoforms have been found for this gene.
Alias Symbols: ADAPT78, CSP1, DSC1, DSCR1, MCIP1, RCN1
Protein Interaction Partner: UBC, PPP3CA, NEDD8, APP, FBXW11, BTRC, FBXW4, TOLLIP, TRAF6, IRAK1, HSP90AA1, STAT2, GSK3B, MAP3K3, MAPK3, UXT,
Protein Size: 252

Molecular Weight: 28 kDa

Gene ID: 1827

NCBI Accession: NM_004414, NP_004405

UniProt: P53805

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development