NRF1 antibody (N-Term)

Catalog No. ABIN2780635

The Rabbit Polyclonal anti-NRF1 antibody has been validated for WB. It is suitable to detect NRF1 in samples from Human, Mouse, Rat, Cow, Rabbit, Horse, Guinea Pig, Zebrafish (Danio rerio) and Dog.

Quick Overview for NRF1 antibody (N-Term) (ABIN2780635)

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Horse, Guinea Pig, Zebrafish (Danio rerio), Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NRF1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NRF1 Antibody

Binding Specificity: N-Term

Sequence: MEEHGVTQTE HMATIEAHAV AQQVQQVHVA TYTEHSMLSA DEDSPSSPED

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against NRF1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NRF1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 503 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NRF1

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Alternative Name: NRF1

Background: NRF1 is a phosphorylated nuclear protein with a bZIP domain. This protein homodimerizes and functions as a transcription factor that activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternate transcriptional splice variants, which encode the same protein, have been characterized. Additional variants encoding different protein isoforms have been described but they have not been fully characterized. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for 'nuclear factor (erythroid-derived 2)-like 1' which has an official symbol of NFE2L1.
Alias Symbols: ALPHA-PAL
Protein Interaction Partner: POGZ, TRAF2, SP4, FHL2, DYNLL1, CSNK2B, CSNK2A1, UBC, HHV8GK18_gp81, CEBPB, PARP1, PPRC1, MAFF, PPARGC1A, Dynlt1b, CDK1, MRPL57, TFAM,
Protein Size: 503

Molecular Weight: 53 kDa

Gene ID: 4899

NCBI Accession: NM_005011, NP_005002

UniProt: Q16656

Pathways: Regulation of Lipid Metabolism by PPARalpha