RBM5 antibody (N-Term)

Catalog No. ABIN2780715

This Rabbit Polyclonal antibody specifically detects RBM5 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig and Saccharomyces cerevisiae.

Quick Overview for RBM5 antibody (N-Term) (ABIN2780715)

Target: RBM5 (RNA Binding Motif Protein 5 (RBM5))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RBM5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RBM5 Antibody

Binding Specificity: N-Term

Sequence: GSDKRVSRTE RSGRYGSIID RDDRDERESR SRRRDSDYKR SSDDRRGDRY

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 80%

Characteristics: This is a rabbit polyclonal antibody against RBM5. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human RBM5

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 815 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for RBM5

Target: RBM5 (RNA Binding Motif Protein 5 (RBM5))

Alternative Name: RBM5

Background: RBM5 is a component of the spliceosome A complex.It regulates alternative splicing of a number of mRNAs. RBM5 may modulate splice site pairing after recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of the intron. RBM5 may both positively and negatively regulate aopotosis by regulating the alternative splicing of several genes involved in this process, including FAS and CASP2/caspase-2. In the case of FAS, promotes exclusion of exon 6 thereby producing a soluble form of FAS that inhibits apoptosis. In the case of CASP2/caspase-2, RBM5 promotes exclusion of exon 9 thereby producing a catalytically active form of CASP2/Caspase-2 that induces apoptosis.
Alias Symbols: FLJ39876, G15, H37, LUCA15, RMB5
Protein Interaction Partner: UBC, RNF2, SUZ12, WDR77, WBP11, LSM8, PRPF19, U2SURP, SNRNP200, U2AF2, PRPF8, ILF3, DHX15, LIG4, COPS5, SF3A2, SUMO2, GAPDH, BCL2L1, LRIF1,
Protein Size: 815

Molecular Weight: 92 kDa

Gene ID: 10181

NCBI Accession: NM_005778, NP_005769

UniProt: P52756

Pathways: Ribonucleoprotein Complex Subunit Organization