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SMAD1 antibody (N-Term)

This anti-SMAD1 antibody is a Rabbit Polyclonal antibody detecting SMAD1 in WB and IHC. Suitable for Human, Mouse, Rat, Cow, Dog, Zebrafish (Danio rerio), Horse, Guinea Pig and Rabbit.
Catalog No. ABIN2780719

Quick Overview for SMAD1 antibody (N-Term) (ABIN2780719)

Target

See all SMAD1 Antibodies
SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Reactivity

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Human, Mouse, Rat, Cow, Dog, Zebrafish (Danio rerio), Horse, Guinea Pig, Rabbit

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This SMAD1 antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC)
  • Binding Specificity

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    N-Term

    Sequence

    MNVTSLFSFT SPAVKRLLGW KQGDEEEKWA EKAVDALVKK LKKKKGAMEE

    Specificity

    This antibody reacts with SMAD1 + SMAD5 and to a lesser extent, SMAD8.

    Predicted Reactivity

    Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

    Characteristics

    This is a rabbit polyclonal antibody against SMAD1. It was validated on Western Blot and immunohistochemistry.

    Purification

    Protein A purified

    Immunogen

    The immunogen is a synthetic peptide directed towards the N terminal region of human SMAD1
  • Application Notes

    Optimal working dilutions should be determined experimentally by the investigator.

    Comment

    Antigen size: 465 AA

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freeze-thaw cycles.

    Storage

    -20 °C

    Storage Comment

    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Target

    SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

    Alternative Name

    SMAD1

    Background

    SMAD1 belongs to the SMAD family. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD1 mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, SMAD1 can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of SMAD1 forms a complex with SMAD4, which is important for its function in the transcription regulation. SMAD1 is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation.The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.
    Alias Symbols: BSP1, JV4-1, JV41, MADH1, MADR1, BSP-1
    Protein Interaction Partner: HDAC1, SMURF1, WDR77, AR, UBC, ACVRL1, SMAD4, LEMD3, MAPK1, GSK3B, ZNF521, ZNF423, AKR1B1, AP2A2, PIAS4, ZDHHC3, HBP1, IRF2BP1, SF3B1, MGA, ZNF510, ICK, GMEB1, ZEB2, PUM1, PIGQ, PIAS1, TTF2, KMT2D, ZNF76, XPC, TTF1, SOX5, SNRNP70, SMAD3, SMAD2, SMAD1, INP
    Protein Size: 465

    Molecular Weight

    52 kDa

    Gene ID

    4086

    NCBI Accession

    NM_005900, NP_005891

    UniProt

    Q99717

    Pathways

    Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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