PDLIM5 antibody (C-Term)

Catalog No. ABIN2780776

The Rabbit Polyclonal anti-PDLIM5 antibody has been validated for WB. It is suitable to detect PDLIM5 in samples from Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig and Zebrafish (Danio rerio).

Quick Overview for PDLIM5 antibody (C-Term) (ABIN2780776)

Target: PDLIM5 (PDZ and LIM Domain 5 (PDLIM5))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDLIM5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PDLIM5 Antibody

Binding Specificity: C-Term

Sequence: AGDMFLEALG YTWHDTCFVC SVCCESLEGQ TFFSKKDKPL CKKHAHSVNF

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against PDLIM5. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human PDLIM5

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 596 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PDLIM5

Target: PDLIM5 (PDZ and LIM Domain 5 (PDLIM5))

Alternative Name: PDLIM5

Background: PDLIM5 is a LIM domain protein. LIM domains are cysteine-rich double zinc fingers composed of 50 to 60 amino acids that are involved in protein-protein interactions. LIM domain-containing proteins are scaffolds for the formation of multiprotein complexes. The proteins are involved in cytoskeleton organization, cell lineage specification, organ development, and oncogenesis. The encoded protein is also a member of the Enigma class of proteins, a family of proteins that possess a 100-amino acid PDZ domain in the N terminus and 1 to 3 LIM domains in the C terminus. Multiple transcript variants encoding different isoforms have been found for this gene, although not all of them have been fully characterized.The protein encoded by this gene is a LIM domain protein. LIM domains are cysteine-rich double zinc fingers composed of 50 to 60 amino acids that are involved in protein-protein interactions. LIM domain-containing proteins are scaffolds for the formation of multiprotein complexes. The proteins are involved in cytoskeleton organization, cell lineage specification, organ development, and oncogenesis. The encoded protein is also a member of the Enigma class of proteins, a family of proteins that possess a 100-amino acid PDZ domain in the N terminus and 1 to 3 LIM domains in the C terminus. Multiple transcript variants encoding different isoforms have been found for this gene, although not all of them have been fully characterized.
Alias Symbols: L9, ENH, LIM, ENH1
Protein Interaction Partner: KRTAP10-7, KRT40, CCDC136, COIL, ZYX, UBC, SUMO2, SUMO3, SUZ12, RNF2, BMI1, PDE12, NPLOC4, IPO11, PLIN3, UBA2, EIF4A3, ANP32A, TPD52, SRP14, RAD23B, PKM, METTL1, CBS, CALU, FN1, ELMSAN1, SIN3A, DSTN, NDUFA7, GFI1B, SUMO1, TRIM39, CACNA1B, PRKCG, PRKCE, PR
Protein Size: 596

Molecular Weight: 64 kDa

Gene ID: 10611

NCBI Accession: NM_006457, NP_006448

UniProt: Q5UW38