Interleukin enhancer-binding factor 3 (ILF3) (C-Term) antibody

Catalog No. ABIN2780868

The Rabbit Polyclonal anti- antibody has been validated for WB. It is suitable to detect in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit and Horse.

Quick Overview for Interleukin enhancer-binding factor 3 (ILF3) (C-Term) antibody (ABIN2780868)

Target: Interleukin enhancer-binding factor 3 (ILF3)

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Binding Specificity: C-Term

Sequence: SYQGKQGGYS QSNYNSPGSG QNYSGPPSSY QSSQGGYGRN ADHSMNYQYR

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 79%, Rabbit: 93%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against ILF3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human ILF3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 894 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details

Target: Interleukin enhancer-binding factor 3 (ILF3)

Alternative Name: ILF3

Background: ILF3 may facilitate double-stranded RNA-regulated gene expression at the level of post-transcription. ILF3 can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. ILF3 can regulate protein arginine N-methyltransferase 1 activity. ILF3 may regulate transcription of the IL2 gene during T-cell activation. It can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of interleukin 2. NFAT binds to a sequence in the IL2 enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the larger of which is the product of this gene. The encoded protein, which is primarily localized to ribosomes, probably regulates transcription at the level of mRNA elongation. At least three transcript variants encoding three different isoforms have been found for this gene.
Alias Symbols: CBTF, DRBF, MMP4, MPP4, NF90, NFAR, NF110, NF90a, NF90b, NFAR2, TCP80, DRBP76, NF110b, NFAR-1, TCP110, MPHOSPH4, NF-AT-90
Protein Interaction Partner: PLSCR1, UBC, FBXW11, RBPMS, HUWE1, MDM2, AURKA, SUMO2, SUMO3, STAU1, IVNS1ABP, LGR4, ERG, RPA3, RPA2, RPA1, EZH2, HNRNPL, SUZ12, RNF2, BMI1, EED, VHL, VEGFA, rev, FBXO6, TARDBP, CSF2, EIF2AK2, ICAM1, IGSF8, SNORD116-22, PABPC1, RNU2-4P, RNU5B-1, RNU5A-1,
Protein Size: 894

Molecular Weight: 95 kDa

Gene ID: 3609

NCBI Accession: NM_012218, NP_036350

UniProt: Q12906

Pathways: M Phase